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PRISM Wet-Lab Protocol

Safety First

Always wear appropriate PPE when handling reagents, especially PFA and formamide.

Shared Protocol

The PRISM wet-lab workflow follows the standard RCA protocol for the shared preprocessing steps (fixation through post-amplification). See the full bench-ready SOPs:

PRISM-Specific Steps

After the shared RCA protocol, PRISM uses single-round fluorescence imaging with radius vector encoding:

  1. Fluorescent Probe Hybridization: Hybridize detection probes carrying unique fluorescent barcodes to the RCA products.
  2. Single-Round Imaging: Capture all barcode signals in a single imaging round across multiple fluorescence channels.
  3. Decoding: Use radius vector encoding to decode gene identity from the fluorescent barcode pattern.